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mix n staintm cf dye antibody labeling kits  (Biotium)


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    Structured Review

    Biotium mix n staintm cf dye antibody labeling kits
    Mix N Staintm Cf Dye Antibody Labeling Kits, supplied by Biotium, used in various techniques. Bioz Stars score: 95/100, based on 95 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mix+n+stain+cf+dye+antibody+labeling+kit/Mix-n-Stain+Biotin+Antibody+Labeling+Kit/pm42020418-203-17-34
    Average 95 stars, based on 95 article reviews
    mix n staintm cf dye antibody labeling kits - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    other:

    Article Title: A Conserved Domain of Cfap298 Governs Left-Right Symmetry Breaking in Vertebrates
    Article Snippet: Acetylated-tubulin primary antibody was directly conjugated with Alexa 555 fluorophore using Biotium Mix-n-StainTM CF® Dye Antibody Labeling Kit (#92274), and gamma-tubulin primary antibody was conjugated with Alexa 647 fluorophore using Biotium Mix-n-StainTM CF® Dye Antibody Labeling Kit (#92274).

    Article Title: Immuno-functionomics reveals geographical variation and a role for TLR8 in mRNA vaccine responses
    Article Snippet: Cat.368502) using Mix-n-StainTM CF® Dye Antibody Labeling Kit (Biotium, Cat.92235), CD45-PacificBlue (Biolegend, Cat.368540) and/or CD45-AlexaFluor700 (Biolegend, Cat.368514) for 15 minutes at RT, followed by washing with FACS buffer (Phosphate buffered saline (PBS) containing 2 % (v/v) BSA, 2 mM EDTA) ( ).

    Article Title: Dual Targeting for Enhanced Tumor Immunity: Conditionally Active CD28xVISTA Bispecific Antibodies Promote Myeloid-Driven T-Cell Activation
    Article Snippet: Binding of serial diluted CF647-labelled antibodies (Mix-n-StainTM CF® Dye Antibody Labeling Kit, Biotium Cat. #92238) to native proteins on Jurkat T-cells (CD28), Kasumi-3 (VISTA) or a CHO cell line overexpressing VISTA were analyzed by flow cytometry.

    Antibody Labeling:

    Article Title: Humanization of CD47 enables development of functional human neutrophils via postirradiation remodeling of the bone marrow
    Article Snippet: .. Anti-Histone H3 (citrulline R2 + R8 + R17) antibody from Abcam (RM1001) was conjugated using Biotium Mix-n-Stain CF Dye Antibody Labeling Kit following manufacturer’s instructions. ..

    Article Title: Quantitative 3D imaging of mouse and human intrahepatic bile ducts in homeostasis and liver injury
    Article Snippet: Samples were blocked for 2d at RT in Blocking Solution [84% PTx.2, 6% NDS (Jackson Immuno; 017-000-121), 10% DMSO]. .. Samples were stained with 2.5μg/mL anti-CD326 antibody conjugated to 647nm for mouse (BioLegend; 118212) or 2.5μg/mL anti-CD326 antibody (BioLegend; 324202) in-house conjugated to 750nm using Mix-n-Stain CF Dye Antibody Labeling Kit (Biotium; 92241) for human. .. Conjugated antibodies were diluted in PTwH [0.002% Tween-20 and 4 μg/mL heparin (Sigma; H3393 in PBS]/ 5%DMSO/3% NDS and for 7d.

    Article Title: Orderly mitosis shapes interphase genome architecture
    Article Snippet: Anti-CENP-C (MBL Biosciences, Cat. No. PD030) and anti-CENP-A (Abcam, Cat. No. AB13939) antibodies were diluted 1:1000 in blocking buffer (5% BSA solution prepared in PBST) and used for IF staining. .. In experiments where cells were EdU labelled, CENP-C primary antibody was directly conjugated with fluorophores using Mix-n-Stain CF Dye Antibody Labeling Kit (Biotium, Cat. No. 922235). .. Anti-FLAG monoclonal antibody (Sigma, cat. No. F3165250) was diluted 1:250 in blocking buffer and used for IF staining.

    Article Title: A conserved domain of Cfap298 governs left–right symmetry breaking in vertebrates
    Article Snippet: The following primary and secondary antibodies used: guinea pig anti-Celsr1 (1:1000; ), mouse anti-acetylated-tubulin (1:200; Sigma, T6793), mouse anti-γ-tubulin (1:200; Sigma, T6557), rat anti-P-cadherin (1:250; Invitrogen, 13-2000z), rabbit anti-Sox9 (1:1000; Millipore, AB5535), Alexa Fluor 488 donkey anti-guinea pig (1:1000; Jackson Immuno, 706-545-148). .. Anti-acetylated-tubulin primary antibody was directly conjugated with Alexa 555 fluorophore using Biotium Mix-n-Stain CF Dye Antibody Labeling Kit (92274), and anti-gamma-tubulin primary antibody was conjugated with Alexa 647 fluorophore using Biotium Mix-n-Stain CF Dye Antibody Labeling Kit (92274). ..

    Article Title: Quantitative 3D Imaging of Mouse and Human Intrahepatic Bile Ducts in Homeostasis and Liver Injury
    Article Snippet: Samples were blocked for 2 days at room temperature in Blocking Solution [84% PTx.2, 6% normal donkey serum (017-000-121; Jackson ImmunoResearch Laboratories), 10% dimethyl sulfoxide]. .. Samples were stained with 2.5 μg/mL anti-CD326 antibody conjugated to Alexa Fluor 647 for mouse (118212; BioLegend) or 2.5 μg/mL anti-CD326 antibody (324202; BioLegend) in-house conjugated to Alexa Fluor 750 using a Mix-n-Stain CF Dye Antibody Labeling Kit (92241; Biotium, Fremont, CA) for human. .. Conjugated antibodies were diluted in PTwH [0.002% Tween-20 and 4 μg/mL heparin (H3393 in PBS; MilliporeSigma)]/5% dimethyl sulfoxide/3% normal donkey serum and for 7 days.

    Staining:

    Article Title: Quantitative 3D imaging of mouse and human intrahepatic bile ducts in homeostasis and liver injury
    Article Snippet: Samples were blocked for 2d at RT in Blocking Solution [84% PTx.2, 6% NDS (Jackson Immuno; 017-000-121), 10% DMSO]. .. Samples were stained with 2.5μg/mL anti-CD326 antibody conjugated to 647nm for mouse (BioLegend; 118212) or 2.5μg/mL anti-CD326 antibody (BioLegend; 324202) in-house conjugated to 750nm using Mix-n-Stain CF Dye Antibody Labeling Kit (Biotium; 92241) for human. .. Conjugated antibodies were diluted in PTwH [0.002% Tween-20 and 4 μg/mL heparin (Sigma; H3393 in PBS]/ 5%DMSO/3% NDS and for 7d.

    Article Title: Quantitative 3D Imaging of Mouse and Human Intrahepatic Bile Ducts in Homeostasis and Liver Injury
    Article Snippet: Samples were blocked for 2 days at room temperature in Blocking Solution [84% PTx.2, 6% normal donkey serum (017-000-121; Jackson ImmunoResearch Laboratories), 10% dimethyl sulfoxide]. .. Samples were stained with 2.5 μg/mL anti-CD326 antibody conjugated to Alexa Fluor 647 for mouse (118212; BioLegend) or 2.5 μg/mL anti-CD326 antibody (324202; BioLegend) in-house conjugated to Alexa Fluor 750 using a Mix-n-Stain CF Dye Antibody Labeling Kit (92241; Biotium, Fremont, CA) for human. .. Conjugated antibodies were diluted in PTwH [0.002% Tween-20 and 4 μg/mL heparin (H3393 in PBS; MilliporeSigma)]/5% dimethyl sulfoxide/3% normal donkey serum and for 7 days.



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    Image Search Results


    Journal: iScience

    Article Title: Immuno-functionomics reveals geographical variation and a role for TLR8 in mRNA vaccine responses

    doi: 10.1016/j.isci.2025.113839

    Figure Lengend Snippet:

    Article Snippet: Mix-n-StainTM CF® Dye Antibody Labeling Kit , Biotium , Cat#92235.

    Techniques: Staining, Recombinant, Saline, Antibody Labeling, Binding Assay, Software, Cytometry